Feasibility of Somatic Embryo Development in Musa, cv. Bluggoe

Abstract

The uppermost part of proliferating buds (in vitro) of Musa, ABB, cv. Bluggoe, produced meristematic globules in basic Murashige and Skoog liquid medium (Murashige and Skoog, 1062), supplemented with 2,4 D (5.10~6 M) and BAP (I0~6 M). Individual meristematic globules of size < = 2 mm diameter were confirmed as somatic pro - embryos. Somatic embryos were obtained upon successive transfer of pro-r embryos initially into a medium with ABA (10~5 M), and then to a medium without any growth regulators and finally to BAP (10~6 M) and IAA (I0~6 M). Tlie embryo proper (shoot and root apex) and continuous pro^ cambial strands between the shoot and root apices were observed in these, structures.