IN VITRO CALLUS AND C E L L CULTURES OF GOSSYPIUM HIRSUTUM L. (COTTON)

Abstract

The leaf, petiole, green stem, immature seed and anthers of Gossypium hirsutum L. (cotton) were cultured on the basic Murashige and Skoog (1962) medium (MS), supplemented with different types of growth regulators at varying concentrations. They were cultured in 24 h light and dark conditions separately. Callus formation was observed only on leaf and mature seed explants cultured in light. The morphological and histological characters of the two calluses varied. The calluses were transferred separately to basic MS liquid medium supplemented with 2,4-Dichlorophenoxy acetic acid (2,4-D) and/or Benzyl amino purine (BAP), to develop cell cultures. Cellular and quantitative studies on the cell cultures enabled selection of the most suitable culture for plant regeneration.