Formation of an Ointment from the Herbal Sources of Nikadi Pottani and Identification the Contribution of Each Source toward the Active Ingredients of the Final Product with TLC Finger Prints

Abstract

There is a tendency of increasing of neurological and inflammatory disorders in society. Thus, the study was carried to prepare a user friendly Analgesic, Anti-inflammatory ointment from the traditional Ayurvedic Thavili Pottani used for neurological and inflammatory disorders. Contribution of active ingredients from each plant source was also tested with TLC fingerprints. Study was carried in Dravyaguna laboratory (April, 2019 to April, 2020). Dried plant parts of Vitex nigundo, Adhatoda vasica, Ricinus communis, Alpinia galanga, Brassica nigra, Allium sativum were fine powdered and mixed up. The mixture was soxhlet extracted with ethanol. The extract was mixed-up with ointment base containing bee`s wax, liquid paraffin, methyl paraben and propyl paraben and prepared the ointment. Secondary, the powdered mixture as well as each powdered plant material was soxhlet extracted ethanol separately and concentrated with rotary evaporator. Eventually a 10 µl of each was placed in a same TLC plate and run in a chamber (mobile phase = Toluene: Ethyl acetate = 13:1). Under TLC fingerprints, visible bands were found for the mixture (six) as well as each plant source. Out of the six bands of mixture, two were tallied with the two bands of Vitex, and Alpinia. Three bands were tallied with three bands of Adhatoda, and Ricinus. One band was tallied with a band of Brassica. Hence, among the ingredients of final product, highest contribution was from Adhatoda, and Ricinus. Second most was from Vitex, and Alpinia and third most contribution was from Brassica. The contribution from Allium was invisible and this could be due to volatile nature of the ingredients.