Purification of an acidic coniferin-hydrolysing β-glucosidase from developing xylem of Pinus banksiana

dc.contributor.authorLeinhos, Volker
dc.contributor.authorUdagama-Randeniya, Preethi V.
dc.contributor.authorSavidge, Rodney A.
dc.date.accessioned2021-06-05T10:13:57Z
dc.date.available2021-06-05T10:13:57Z
dc.date.issued1994
dc.description.abstractTwo cytosolic, acidic (pI 3.8) glycoproteins (Mr 110 000 and 90 000) from lignifying xylem of Pinus banksiana were electrophoretically isolated and confirmed by combined gas chromatography-mass spectrometry to be capable of hydrolysing E-coniferin to trans-coniferyl alcohol. These isoenzymes yielded identical N-terminal sequences having negligible homology with documented glucosidases, suggesting that they constitute a novel form of β-glucosidase.en_US
dc.description.sponsorshipis work was supported by the Natural Sciences and Engineering Research Council of Canada and by Forestry Canada.en_US
dc.identifier.citation38en_US
dc.identifier.otherhttps://doi.org/10.1016/0031-9422(94)85053-4
dc.identifier.urihttp://archive.cmb.ac.lk:8080/xmlui/handle/70130/5232
dc.language.isoenen_US
dc.publisherPhytochemistryen_US
dc.subjectPinus banksianaen_US
dc.subjectGymnospermaeen_US
dc.subjectcambial growthen_US
dc.subjectbeta-glucosidaseen_US
dc.subjectligninen_US
dc.subjectsecondary wall developmenten_US
dc.subjecttracheary element differentiationen_US
dc.subjectwood formationen_US
dc.subjectxylogenesisen_US
dc.titlePurification of an acidic coniferin-hydrolysing β-glucosidase from developing xylem of Pinus banksianaen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
13.pdf
Size:
756.76 KB
Format:
Adobe Portable Document Format
Description:
Main article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: