Optimization of initial nanobiotechnology procedures to design a gold nanoparticle-based nucleic acid lateral flow assay to detect BRAF V600E mutation in papillary thyroid carcinoma
Loading...
Files
Date
Journal Title
Journal ISSN
Volume Title
Publisher
University of Colombo
Abstract
Introduction: Lateral flow assays (LFAs) have significant applications in point-of-care detection of cancer biomarkers. Proper validation of each step in their development process is crucial in achieving the final outcome. This study aimed to develop optimised protocols to design a gold nanoparticle (AuNP)-based nucleic acid lateral flow assay (NALFA) to detect the BRAF V600E mutation for prognostication of papillary thyroid carcinoma (PTC). Methods: Design of primers for the multiplex polymerase chain reaction (PCR) and oligonucleotide probes for LFA and optimization of multiplex PCR to amplify fragments of BRAF and GAPDH were carried out. Characterization of AuNPs, evaluation of their stability over time and assessment of their conjugation to detection probes (DPs) were performed.
Description
Keywords
BRAF V600E, gold nanoparticle-based nucleic acid lateral flow assay, multiplex polymerase chain reaction, papillary thyroid carcinoma
Citation
Wijesuriya, N. D., Wijesundera, W. S. S., Thoradeniya, S. T., Jayasundara, D. R., Priyani, A. A. H., Jayathilaka, L. D., & Siribaddana, P. (2022). Optimization of initial nanobiotechnology procedures to design a gold nanoparticle-based nucleic acid lateral flow assay to detect BRAF V600E mutation in papillary thyroid carcinoma. Proceedings of the Annual Research Symposium-2022, University of Colombo, Sri Lanka, p. 199.