Development of a diagnostic dna probe to detect setaria digtata: The causative parasite of cerebrospinal nematodiasis in goats,sheep and horses

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dc.contributor.authorWijesundera, W.S.S.
dc.contributor.authorChandrasekharan, N.V.
dc.contributor.authorKarunanayake, E.H.
dc.contributor.authorDarmasena, S.P.
dc.date.accessioned2021-07-30T04:33:54Z
dc.date.available2021-07-30T04:33:54Z
dc.date.issued1995
dc.description.abstractTwo repetitive sequences (IpSdM and IpSdS) have been cloned andsequenced from the genome of Setaria digitata. When IpSdM (214 bp) and IpSdS (201 bp) were aligned, a high degree of homology (85%) was observed, indicating that they belong to the same family of repeats. IpSdM represents a complete repeating element while IpSdS consists of two partial repeating elements arranged in tandem. The elements are present in about 10 000 copies comprising 2.8% of the S. digilata genome. As a diagnostic probe IpSdM detects as little as 100 pg DNA of both S. digilata and S. labiato-papillosa. It can also detect a single microfilaria and a L3 larva making it a valuable tool to monitor cattle and mosquito vector populations in the prevention of cerebrospinal nematodiasis.en_US
dc.identifier.citationWijesundera, W. S. S., Chandrasekharan, N. V., Karunanayake, E. H., & Dharmasena, S. P. (1996). Development of a diagnostic DNA probe to detect Setaria digitata: the causative parasite of cerebrospinal nematodiasis in goats, sheep and horses. British Veterinary Journal, 152(5), 561-571.en_US
dc.identifier.urihttp://archive.cmb.ac.lk/handle/70130/5603
dc.publisherElsevieren_US
dc.subjectSetaria digilataen_US
dc.subjectrepetitive sequencesen_US
dc.subjecttandem repeatsen_US
dc.subjectDNA probesen_US
dc.subjectcerebrospinal nematodiasisen_US
dc.titleDevelopment of a diagnostic dna probe to detect setaria digtata: The causative parasite of cerebrospinal nematodiasis in goats,sheep and horsesen_US
dc.typeArticleen_US

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