A Rapid Method for the Separation of Protein-Bound and Ionized Calcium in Human Serum and the Establishment of the Clinical Norm for Ceylon Subjects

Abstract

The calcium in the plasma water is distributed in three forms viz . ionized (Ca + + ) protein bound (PB-Ca) and complexed. (Fanconi & Rose, 1958; Rose, 1957). The accepted methods o f determining the ionized calcium (Ca++) o f plasma are the biological method (McLean & Hastings, 1934), and the chemical method (Rose, 1957). The use of ion exchange resins is another procedure (Slade, 1956). Gel filtration with cross linked dextrans has been used widely in recent times for the separation o f protein components from salts and low molecular weight compounds (Porath & Flodin, 1959; Kisliuk, i960; Flodin, 1961). Recently, Jacobson and Widstro m (1962) described a very simple method for the separation o f protein bound iodine components from non-protein ones. The method o f Jacobson and Widstrom (1962) when applied for PB-Ca and Ca + + separations did not yield satisfactory results. In this paper, a method is described to separate these two fractions. Gel filtration with cross linked dextran is introduced as a very simple and rapid method for the separation of both the PB-C a and Ca++.The calcium in the plasma water is distributed in three forms viz . ionized (Ca + + ) protein bound (PB-Ca) and complexed. (Fanconi & Rose, 1958; Rose, 1957). The accepted methods o f determining the ionized calcium (Ca++) o f plasma are the biological method (McLean & Hastings, 1934), and the chemical method (Rose, 1957). The use of ion exchange resins is another procedure (Slade, 1956). Gel filtration with cross linked dextrans has been used widely in recent times for the separation o f protein components from salts and low molecular weight compounds (Porath & Flodin, 1959; Kisliuk, i960; Flodin, 1961). Recently, Jacobson and Widstro m (1962) described a very simple method for the separation o f protein bound iodine components from non-protein ones. The method o f Jacobson and Widstrom (1962) when applied for PB-Ca and Ca + + separations did not yield satisfactory results. In this paper, a method is described to separate these two fractions. Gel filtration with cross linked dextran is introduced as a very simple and rapid method for the separation of both the PB-C a and Ca++.