The design and implementation of a novel pharmacogenomic assay to study the distribution of CYP3A5*3 (rs776746) and CYP3A5*1E (rs4646453) polymorphisms and its implications to predict the response to the immunosuppressive drug tacrolimus

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dc.contributor.authorSameem, R.
dc.contributor.authorDissanayake, V.H.W.
dc.contributor.authorPraveenan, S.
dc.contributor.authorWetthasinghe, K.T.
dc.contributor.authorNoordeen, N.
dc.date.accessioned2026-09-03T15:29:26Z
dc.date.issued2022
dc.description.abstractIntroduction: Tacrolimus is a first-line immunosuppressive agent for solid organ transplantations. Due to its narrow therapeutic index, tacrolimus demonstrates a wide inter individual variability in pharmacokinetics. Genetic polymorphisms in CYP3A5 are significant determinants of tacrolimus efficacy. Methods: A total of 100 healthy individuals from the Sri Lankan population were screened for the CYP3A5 single nucleotide polymorphisms (SNPs), CYP3A5*3 (rs776746) and CYP3A5*1E (rs4646453) using a novel single variant tetra-primer amplification refractory mutation system (ARMS) polymerase chain reaction (PCR) assay and the results were validated by DNA sequencing
dc.identifier.citationSameem, R., Noordeen, N., Wetthasinghe, K. T., Praveenan, S., & Dissanayake, V. H. W. (2022). The design and implementation of a novel pharmacogenomic assay to study the distribution of CYP3A53 (rs776746) and CYP3A51E (rs4646453) polymorphisms and its implications to predict the response to the immunosuppressive drug tacrolimus. Proceedings of the Annual Research Symposium-2022, University of Colombo, Sri Lanka, p. 202.
dc.identifier.urihttps://archive.cmb.ac.lk/handle/70130/9115
dc.language.isoen
dc.publisherUniversity of Colombo
dc.subjecttacrolimus
dc.subjectCYP3A5
dc.subjecttetra-primer ARMS polymerase chain reaction
dc.subjectpharmacogenomic assay
dc.titleThe design and implementation of a novel pharmacogenomic assay to study the distribution of CYP3A5*3 (rs776746) and CYP3A5*1E (rs4646453) polymorphisms and its implications to predict the response to the immunosuppressive drug tacrolimus
dc.typeArticle

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