Abstract:
The anther culture ability of two local indica rice varieties were evaluated by anther plating on N6 and
SK-I callus induction media. The traditional rice variety, Kurulu Thuda displayed good anther culture
potential with a callus induction frequency of 17.2%. Anthers most suitable for culture were those bearing
bi-nucleate pollen. Anther response was relatively poor in the new improved variety, Bg 250 (1.4%).
Callus induction was nearly three times higher on N6 medium than on SK-I medium in var. Kurulu
Thuda. Shoot regeneration was achieved in var. Kurulu Thuda on SK-II medium, but only from callus
induced on N6 medium. From a total of 115 calli transferred from N6 to regeneration medium shoot
development occurred in 7 calli, thus limiting the overall anther culture efficiency. Of the calli with
morphogenetic competence, 5 gave rise to albino shoots and 2 produced green shoots. After root development
the green plantlets were potted in soil. Plant regeneration could not be observed from callus
induced in var. Bg 250.
