Optimized PEI Mediated Reverse Transfection Methodology for Transient Transfection of HEK293 Cells

Abstract

Non-viral vectors are increasingly used for gene delivery due to their safety and flexibility. Polyethyleneimine (PEI) is widely applied in transfection, but high PEI and DNA concentrations can induce cytotoxicity, necessitating careful optimization of the PEI ratio. ...