Abstract:
Ayurvedic and traditional oils play a major role in internal and external treatment for
numerous diseases as well as wellness in Ayurveda. Ayurveda studies have gifted
thousands of oil recipes with dynamic applications. Seethodaka, Neelyadi, and
Visarpahara are some of the commonly available traditional oils which show
wondering effects in treating skin diseases such as ulcers, wounds, rashes and boils.
Staphylococcus aureus and Escherichia coli are the most widely detected bacteria in
skin conditions. This study conveys the determination of the antimicrobial effects of
Seethodaka, Visarpahara, and Neelyadi oils against Staphylococcus aureus and
Escherichia coli using standard antibiotic sensitivity tests. All three oil preparations
were freshly prepared with the combination of appropriate ingredients in ratios
based on the traditional Ayurveda classics. Microbial assays were conducted using
Mueller Hinton Agar (MHA) medium. Five wells were made in each plate, and the
bottom was sealed using molten agar. Tetracycline (2000 ppm:100 μ L) and DMSO
(100 μ L) were used as positive and negative controls, respectively. Three oil samples,
along with positive and negative controls, were each added 100 μL to individual
plates to assess their antibacterial activity against both strains. Subsequently,
inhibition zones were measured after 24 hours of incubation at 37°C. Each
experiment was replicated three times, and the mean inhibition zone diameter was
calculated for each plate. The outcomes revealed a 12 mm Inhibitory zone diameter
for Seethodaka oil against Staphylococcus aureus, whereas Visarpahara oil exhibited
a 16 mm inhibition zone, and Neelyadi oil displayed a 13 mm inhibition zone against
the same bacteria. The positive control, Tetracycline, resulted in a 40 mm inhibition
zone diameter against Staphylococcus aureus. Based on the findings, all three oils
demonstrated significant antimicrobial effect against Staphylococcus aureus.
Moreover, Seethodaka oil exhibited an 8 mm inhibition zone diameter against
Escherichia coli, while Visarpahara oil displayed a remarkable antimicrobial effect
with a 24 mm inhibition zone diameter against Escherichia coli. Neelyadi oil also
indicated substantial inhibition, measuring 12 mm against Escherichia coli. In
contrast, the positive control exhibited a 30 mm inhibition zone diameter against
Escherichia coli. In conclusion, the tested traditional oils, including Seethodaka,
Visarpahara, and Neelyadi, exhibited considerable antibacterial effects against both
Staphylococcus aureus and Escherichia coli.
