Abstract:
Acronychia pedunculata (L.) (Ankenda) under family
RUTACEAE is widely used in Traditional medicine
system. The present study was aimed at the
development of quality control parameters for
identification and authentication of A. pedunculata.
Hence, standardization is important in maintaining
the purity, quality, safety and efficacy of Ayurveda
formulations. Phytochemical, physico-chemical
parameters and HPTLC fingerprints of methanol
extracts of leaves and stem barks were determined as
tools for quality standards. Preliminary
phytochemical analysis revealed the presence of
carbohydrates, reducing sugars, tannins, saponins,
alkaloids, flavonoids, anthranol glycosides, phenols,
terpenoids and proteins in both leaf and stem bark
extracts. Under physico-chemical parameters; total
ash, acid insoluble ash, water soluble ash, loss on
drying and extractability in methanol for A.
pedunculata leaves were 40.38% w/w, 12.64% w/w,
34.42% w/w, 21.80% w/w and 17.84% w/w
respectively. Total ash, acid insoluble ash, water
soluble ash content, loss on drying and extractability
in methanol for A. pedunculata stem barks were
20.42% w/w, 8.35% w/w, 25.56% w/w, 15.67% w/w
and 13.42% w/w. HPTLC profile of A. pedunculata
leaf extract showed 10 peaks (Rf values; -0.01, 0.11,
0.32, 0.39, 0.43, 0.57, 0.69, 0.77, 0.85, 0.88) whereas,
stem bark extract also showed 10 peaks (Rf values; -
0.06, 0.02, 0.06, 0.17, 0.34, 0.48, 0.61, 0.71, 0.82,
0.97) for solvent system; n-hexane: ethyl acetate:
chloroform in 2: 6: 2 ratio. The above parameters can
be considered as a preliminary tool in detection and
contrast of raw materials excluding the counterfeit
and substandard raw materials in Ayurveda
manufacturing.
