Abstract:
Diabetic wounds are a global health issue that has affected many populations worldwide
owing to its severity, slow recovery, and economic burden. Despite the considerable efforts
made in its treatment, they remain a challenge in many nations partly due to its last-resort
treatment of lower-leg amputations, which is burdensome to patients in the long-term. Therefore, the rationale of this study was to explore a topical application prepared in
traditional Sri Lankan medicine as a natural and more cost-effective alternative treatment for
diabetic wounds. The topical application (TA) was prepared into two extracts, TA water and
TA oil, and this study aimed to analyse the wound healing potential of the extracts by means
of their antibacterial and anti-inflammatory activities. The antibacterial activity of both
extracts was determined by performing a disk diffusion assay against four skin pathogens
that inhabit diabetic foot, namely Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus spp., and Escherichia coli with tetracycline antibiotic as the positive control to
obtain the diameter of the zones of inhibition. The anti-inflammatory activity of TA water was
determined using the albumin denaturation method with reference to the known NSAID, Ibuprofen to obtain the percentage inhibition of protein denaturation, which was further
assessed using IC
50 values (GraphPad Prism 9.5.1). The findings indicated that both extracts
possessed sufficient antibacterial potential against all four bacteria. However, in comparison
to tetracycline, the antibacterial activity of the extracts against Pseudomonas aeruginosa was
greater than that of Staphylococcus aureus, Streptococcus spp., and Escherichia coli. Moreover, TA water possessed the highest anti-inflammatory activity at a 0.1 dilution (143.75 mg/ml) of
the concentrated extract (1437.5 mg/ml), which was comparable to that of Ibuprofen (40
mg/ml), and its effectiveness was further confirmed by its IC
50 value (5.14 mg/ml). Therefore, the study concluded that the topical application prepared possesses substantial antibacterial
activity and anti-inflammatory activity validating its potential use in diabetic wound healing
