Abstract:
Introduction: Zingiber zerumbet (L), a member of the family Zingiberaceae, known as “Wal-inguru”
in Sri Lanka is a medicinal herb distributed in Sri Lanka, Bangladesh, India, Malaysia and Nepal. This
plant is used to treat stomach aches, worm infestation in children, swelling sores and loss of appetite in
traditional medicine in Sri Lanka. It has been reported that zerumbone, isolated from rhizome of Z.
zerumbet has reduced the inflammatory response of acute lung injury in endotoxin–treated mice via
Akt-NFkB pathway. Also, zerumbone shows anticancer, anti-inflammatory, anti-ulceration, antioxidant
and antimicrobial properties. Use of Sri Lankan Z. zerumbet in immunomodulatory and lung protection
herbal preparations needs scientifically validated zerumbone content in Sri Lankan Zingiber zerumbet.
Objective: To isolate zerumbone and quantify the content for potential biomedical and clinical
experiments/applications.
Methods: The rhizome and leaf of Z. zerumbet from Nilgala forest of Sri Lanka was hydro-distilled to
obtain its volatile oils and analyzed for volatile oil composition using GC-MS. The essential oil
extracted from the rhizome was purified to isolate zerumbone by size exclusion chromatography using
Sephadex LH20 followed by preparative HPLC. The purified zerumbone was confirmed by 1H-NMR,
13C-NMR and 2D-NMR including HH-COSY, NOESY, DEPT-HSQC and HMBC analysis.
Results: The leaf oil contained 28 compounds in which 26 compounds were identified by GC-MS. It
has trans-nerolidol (41.0%), β-caryophyllene (21.3%) and zerumbone (6.2%) as the major compounds.
The rhizome oil contained 16 compounds of which 14 compounds were identified by GC-MS. The
major components of rhizome oil were zerumbone (68.7%), humulene (12.2%) and camphene (3.6%).
Conclusions: The rhizome oil had 68.7% zerumbone whereas leaf oil had 6.2%. The novel approach
adopted in this study could isolate zermbone with high purity (99.9%) in sufficient quantities for of
zerumbone for potential biomedical applications and lung protection studies.
