Characterization of 6.4 kb repeat from Wucheraria bancrofti

Pelawatte, Thushara Chandanee

dc.contributor.author	Pelawatte, Thushara Chandanee
dc.date.accessioned	2022-03-30T04:09:49Z
dc.date.available	2022-03-30T04:09:49Z
dc.date.issued	1997
dc.identifier.uri	http://archive.cmb.ac.lk:8080/xmlui/handle/70130/6559
dc.description	A thesis submitted in partial fulfilment of the requirements for the degree of Master of Science (Biochemistry, Molecular biology and gene technology) of the University of Colombo, Sri Lanka	en_US
dc.description.abstract	A long repeat of 6.4 kb from the Wuchereria bancrofti genome was partially sequenced, by generating overlapping fragments in pUC 19 with the use of Sau 3A I, Alu I and Rsa I restrication endonucleases. As a result of this, a discontinuous stretch of sequence of 5282 bp was obtained. The longest continuous stretch of nucleotide sequence obtained was 3655 bp. Areas of this long sequence showed 100 percent homology to pWb 2.5, pWb12 and pWb 6 sequences. One end of the pWb 2.5 sequence and one end (5' end) of the 3655 bp sequence showed exact homology. Thus, 2.5 kb fragment of pWb 2.5 is a continuous part of the 6.4 kb repeat and pWb 12 and pWb 6 sequences are interspersed within it. Also, the 6.4 kb repeat is arranged in parasite genome in head-to-tail manner.	en_US
dc.language.iso	en	en_US
dc.publisher	Faculty of Medicine: University of Colombo	en_US
dc.subject	Wuchereria bancrofti	en_US
dc.subject	Wuchereria bancrofti-genetics	en_US
dc.subject	Filariasis	en_US
dc.title	Characterization of 6.4 kb repeat from Wucheraria bancrofti	en_US
dc.type	Thesis	en_US