Mitochondrial-COII sequence polymorphism reflects spatial genetic clustering of Anopheles culicifacies sibling species E in Sri Lanka

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dc.contributor.author Harischandra, I.N.
dc.contributor.author Dassanayake, R.S.
dc.contributor.author de Silva, B.G.D.N.K.
dc.date.accessioned 2021-09-04T07:06:04Z
dc.date.available 2021-09-04T07:06:04Z
dc.date.issued 2021
dc.identifier.citation Mitochondrial-COII sequence polymorphism reflects spatial genetic clustering of Anopheles culicifacies sibling species E in Sri Lanka Harischandra Iresha N, Dassanayake Ranil S, de Silva B.G.D.N.K. Year : 2020 | Volume: 57 | Issue Number: 2 | Page: 139-146 en_US
dc.identifier.uri 10.4103/0972-9062.310864
dc.identifier.uri http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5881
dc.description.abstract Background & objectives: Malaria infects around 216 million people annually with estimated 445,000 deaths globally. Anopheles culicifacies is the vector of malaria in Sri Lanka, a complex of five morphologically identical sibling species of which precise identification using DNA–based methods is still under experimentation. This study was carried out in Sri Lanka to observe the utility of BCE-PCR assay based on mitochondrial Cytochrome Oxidase II (COII) developed in India, in sibling species B and E identification in Sri Lanka, to characterize nucleotide and corresponding amino acid sequences of COII region in major vector sibling species E in Sri Lanka and to analyze the spatial distribution pattern of sibling species E in Sri Lanka using microsatellite markers. Methods: BCE-PCR was carried out for the samples to identify their sibling status. Sequencing of COII region was then carried out to investigate the genetic diversity of Sri Lankan sibling species E, sequences were aligned and compared; microsatellite genotyping was carried out and the spatial clustering pattern was analyzed. Results: Identification of sibling species B and E using BCE-PCR was confusing due to the heterogeneity in the COII region of sibling species in Sri Lanka. Non-synonymous substitutions were detected in COII gene amongst sibling species E. Spatial distributed two clusters were detected in the studied population. Interpretation & conclusion: Existence of genetic variants among sibling species is suggested in Sri Lanka. Further, the pattern of sibling species identification in BCE-PCR was reflected in the spatial clustering of sibling E in Sri Lanka. en_US
dc.language.iso en en_US
dc.publisher Journal of Vector Borne Diseases en_US
dc.subject Anopheles culicifacies sibling E en_US
dc.subject COII en_US
dc.subject microsatellites en_US
dc.subject spatial clustering en_US
dc.title Mitochondrial-COII sequence polymorphism reflects spatial genetic clustering of Anopheles culicifacies sibling species E in Sri Lanka en_US
dc.type Article en_US


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