Efficient targeted mutagenesis in Epichloë festucae using a split marker system

Rahnama, M.; Forester, N.; Ariyawansa, K.G.S.U.; Voisey, C.R.; Johnson, L.J.; Johnson, R.D.; Fleetwood, D.J.

dc.contributor.author	Rahnama, M.
dc.contributor.author	Forester, N.
dc.contributor.author	Ariyawansa, K.G.S.U.
dc.contributor.author	Voisey, C.R.
dc.contributor.author	Johnson, L.J.
dc.contributor.author	Johnson, R.D.
dc.contributor.author	Fleetwood, D.J.
dc.date.accessioned	2021-08-06T03:48:40Z
dc.date.available	2021-08-06T03:48:40Z
dc.date.issued	2017
dc.identifier.citation	M Rahnama, N Forester, K G S U Ariyawansa, C R Voisey, L J Johnson, R D Johnson, D J Fleetwood, Efficient targeted mutagenesis in EpichloÃ« festucae using a split marker system, Journal of Microbiological Methods, Volume 134, 2017, Pages 62-65, ISSN 0167-7012, https://doi.org/10.1016/j.mimet.2016.12.017. (https://www.sciencedirect.com/science/article/pii/S0167701216303682	en_US
dc.identifier.uri	https://doi.org/10.1016/j.mimet.2016.12.017
dc.identifier.uri	http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5690
dc.description.abstract	A split-marker system for targeted gene deletion was developed for the model grass endophytic fungus Epichloë festucae. Compared to the conventional system that yields up to 25% homologous recombinants, the method resulted in 33–74% targeted deletions in E. festucae using as little as 1.5 kb of targeting sequence.	en_US
dc.language.iso	en	en_US
dc.title	Efficient targeted mutagenesis in Epichloë festucae using a split marker system	en_US
dc.type	Article	en_US