Development of a diagnostic dna probe to detect setaria digtata: The causative parasite of cerebrospinal nematodiasis in goats,sheep and horses

Wijesundera, W.S.S.; Chandrasekharan, N.V.; Karunanayake, E.H.; Darmasena, S.P.

dc.contributor.author	Wijesundera, W.S.S.
dc.contributor.author	Chandrasekharan, N.V.
dc.contributor.author	Karunanayake, E.H.
dc.contributor.author	Darmasena, S.P.
dc.date.accessioned	2021-07-30T04:33:54Z
dc.date.available	2021-07-30T04:33:54Z
dc.date.issued	1995
dc.identifier.citation	Wijesundera, W. S. S., Chandrasekharan, N. V., Karunanayake, E. H., & Dharmasena, S. P. (1996). Development of a diagnostic DNA probe to detect Setaria digitata: the causative parasite of cerebrospinal nematodiasis in goats, sheep and horses. British Veterinary Journal, 152(5), 561-571.	en_US
dc.identifier.uri	http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5603
dc.description.abstract	Two repetitive sequences (IpSdM and IpSdS) have been cloned andsequenced from the genome of Setaria digitata. When IpSdM (214 bp) and IpSdS (201 bp) were aligned, a high degree of homology (85%) was observed, indicating that they belong to the same family of repeats. IpSdM represents a complete repeating element while IpSdS consists of two partial repeating elements arranged in tandem. The elements are present in about 10 000 copies comprising 2.8% of the S. digilata genome. As a diagnostic probe IpSdM detects as little as 100 pg DNA of both S. digilata and S. labiato-papillosa. It can also detect a single microfilaria and a L3 larva making it a valuable tool to monitor cattle and mosquito vector populations in the prevention of cerebrospinal nematodiasis.	en_US
dc.publisher	Elsevier	en_US
dc.subject	Setaria digilata	en_US
dc.subject	repetitive sequences	en_US
dc.subject	tandem repeats	en_US
dc.subject	DNA probes	en_US
dc.subject	cerebrospinal nematodiasis	en_US
dc.title	Development of a diagnostic dna probe to detect setaria digtata: The causative parasite of cerebrospinal nematodiasis in goats,sheep and horses	en_US
dc.type	Article	en_US