A sensitive polymerase chain reaction based assay for the detection of Setaria digitata:The causative organism of cerebrospinal nematodiasis in goats,sheep and horses

Wijesundera, W.S.S.; Chandrasekharan, N.V.; Karunanayake, E.H.

dc.contributor.author	Wijesundera, W.S.S.
dc.contributor.author	Chandrasekharan, N.V.
dc.contributor.author	Karunanayake, E.H.
dc.date.accessioned	2021-07-30T04:00:45Z
dc.date.available	2021-07-30T04:00:45Z
dc.date.issued	1998
dc.identifier.citation	Wijesundera, W. S. S., Chandrasekharan, N. V., & Karunanayake, E. H. (1999). A sensitive polymerase chain reaction based assay for the detection of Setaria digitata: The causative organism of cerebrospinal nematodiasis in goats, sheep and horses. Veterinary parasitology, 81(3), 225-233.	en_US
dc.identifier.uri	http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5600
dc.description.abstract	A sensitive PCR assay for the detection of Setaria digitata has been developed. Two oligonucleotide primers (17 nt) were designed from a previously cloned and characterized tandemly arranged repetitive sequence of Setaria digitata. Using these primers, it was possible to amplify small quantities (100 fg) of S. digitata genomic DNA. A simple procedure, using proteinase K and non-ionic detergent NP 40, was followed to process the host blood samples and mosquitoes harbouring L3 larvae. The sensitivity of the polymerase chain reaction based assay surpasses the microscopic detection and the previously reported oligonucleotide based chemiluminescent detection of microfilariae in infected host blood samples and L3 larvae in mosquitoes.	en_US
dc.publisher	Elsevier	en_US
dc.subject	Setaria digitata	en_US
dc.subject	Cerebrospinal nematodiasis	en_US
dc.subject	Diagnosis	en_US
dc.subject	Nematoda	en_US
dc.subject	PCR	en_US
dc.title	A sensitive polymerase chain reaction based assay for the detection of Setaria digitata:The causative organism of cerebrospinal nematodiasis in goats,sheep and horses	en_US
dc.type	Article	en_US