Molecular characterization and comparative expression analysis of two teleostean pro-inflammatory cytokines, IL - 1β and IL-8, from sebastes schlegeli

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dc.contributor.author Herath, H. M. L. P. B
dc.contributor.author Elvitigala, D. A. S
dc.contributor.author Godahewa, G.L
dc.contributor.author Umasuthan, N
dc.contributor.author Whang, I.
dc.contributor.author Noh, J.K.
dc.contributor.author Lee, J
dc.date.accessioned 2021-06-06T08:54:40Z
dc.date.available 2021-06-06T08:54:40Z
dc.date.issued 2016
dc.identifier.citation Herath, H. M. L. P. B., Elvitigala, D. A. S., Godahewa, G. I., Umasuthan, N., Whang, I., Noh, J. K., & Lee, J. (2016). Molecular characterization and comparative expression analysis of two teleostean pro-inflammatory cytokines, IL-1β and IL-8, from Sebastes schlegeli. Gene, 575(2), 732-742. en_US
dc.identifier.uri http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5308
dc.description.abstract Interleukin 1β (IL-1β) and interleukin 8 (IL-8) are two major pro-inflammatory cytokines which play a central role in initiation of inflammatory responses against bacterial- and viral-infections. IL-1β is a member of the interleukin 1 family proteins and IL-8 is classified as a CXC-chemokine. In the current study, putative IL-1β and IL-8 counterparts were identified from a black rockfish transcriptomic database and designated as RfIL-1β and RfIL-8. The RfIL-1β cDNA sequence consists of 1140 nucleotides with a 759 bp open reading frame (ORF) which encodes a 252 amino acid (aa) protein, whereas the RfIL-8 cDNA sequence (898 bp) harbors a 300 bp ORF encoding a 99 aa protein. Furthermore, the RfIL-1β aa sequence contains an IL-1 super family-like domain and an N-terminal IL-1 super family propeptide, while the amino acid sequence of RfIL-8 consists of a typical chemokine-CXC domain. Analysis of sequenced BAC clones containing RfIL-1β and RfIL-8 showed each gene to contain 4 exons interrupted by 3 introns. Pairwise comparison and phylogeny analysis of these cytokine sequences clearly revealed their closer relationship with other corresponding members of teleosts compared to birds and mammals. Constitutive differences in RfIL-1β and RfIL-8 mRNA expression were detected in a tissuespecific manner with the highest expression of each mRNA in spleen tissue. Two immune challenge experiments were conducted with Streptococcus iniae and polyinosinic:polycytidylic acid (poly I:C; a viral double stranded RNA mimic), and transcripts were quantified in spleen and peripheral blood cells. Significantly increased RfIL-1β and RfIL8 transcript levels were detected with almost similar profile patterns, further suggesting a putative involvement of these pro-inflammatory cytokines in the rockfish immunity
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.subject Pro-inflammatory cytokines en_US
dc.subject Immune responses en_US
dc.subject Genomic arrangement en_US
dc.subject Black rockfish en_US
dc.title Molecular characterization and comparative expression analysis of two teleostean pro-inflammatory cytokines, IL - 1β and IL-8, from sebastes schlegeli en_US
dc.type Article en_US


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