Characterization and cytoprotective properties of Sargassum natans fucoidan against urban aerosol-induced keratinocyte damage

Fernando, I. P. Shanura; Sanjeewa, Kalu Kapuge Asanka; Lee, Hyo Geun; Kim, Hyun-Soo; Vaas, Andaravaas Patabadige Jude Prasann; De Silva, H Ireshika Chathurani; Nanayakkara, C.M.; Abeytunga, D Thusitha Udayangani; Lee, Won  Woo; Lee, Dae-Sung; Jeon, You-Jin

dc.contributor.author	Fernando, I. P. Shanura
dc.contributor.author	Sanjeewa, Kalu Kapuge Asanka
dc.contributor.author	Lee, Hyo Geun
dc.contributor.author	Kim, Hyun-Soo
dc.contributor.author	Vaas, Andaravaas Patabadige Jude Prasann
dc.contributor.author	De Silva, H Ireshika Chathurani
dc.contributor.author	Nanayakkara, C.M.
dc.contributor.author	Abeytunga, D Thusitha Udayangani
dc.contributor.author	Lee, Won Woo
dc.contributor.author	Lee, Dae-Sung
dc.contributor.author	Jeon, You-Jin
dc.date.accessioned	2021-06-05T10:29:56Z
dc.date.available	2021-06-05T10:29:56Z
dc.date.issued	2020
dc.identifier.citation	I. P. Shanura Fernando, Kalu Kapuge Asanka Sanjeewa, Hyo Geun Lee, Hyun-Soo Kim, Andaravaas Patabadige Jude Prasanna Vaas, H Ireshika Chathurani De Silva, Chandrika Malkanthi Nanayakkara, D Thusitha Udayangani Abeytunga, Won Woo Lee, Dae-Sung Lee, You-Jin Jeon (2020). Characterization and cytoprotective properties of Sargassum natans fucoidan against urban aerosol-induced keratinocyte damage, International Journal of Biological Macromolecules, Volume 159,15 pp 773-781	en_US
dc.identifier.uri	https://doi.org/10.1016/j.ijbiomac.2020.05.132
dc.identifier.uri	http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5269
dc.description.abstract	The escalation of fine particulate matter (PM) air pollution has recently become a global concern. Evidence is fast accumulating on PM exposure-related skin damage. The present study explored the therapeutic potentials of fucoidan purified from Sargassum natans against damaging effects of PM exposure on human HaCaT keratinocytes. Fucoidan (SNF7) was purified from S. natans by an enzyme-assisted extraction and purified by anion exchange chromatography. SNF7 (≈50 kDa) was identified as a fucoidan containing 70.97% fucose and 36.41 ± 0.59% of sulfate. Treatment of fine dust from Beijing, China (CFD) increased intracellular ROS levels in HaCaT cells triggering DNA damage and apoptosis. Treatment of SNF7 dose-dependently attenuated CFD- induced surge of intracellular ROS levels in keratinocytes by increasing antioxidant defense enzymes. Moreover, SNF7 chelated metal ions Pb2+, Ba2+, Sr2+, Cu2+, Fe2+, and Ca2+ coming from CFD. The results substantiated the potential therapeutic effects of SNF7 against CFD-induced oxidative stress. Further studies could promote SNF7's use as an active ingredient in cosmetics.	en_US
dc.language.iso	en	en_US
dc.subject	Fucoidan, Sargassum natans, Fine dust	en_US
dc.title	Characterization and cytoprotective properties of Sargassum natans fucoidan against urban aerosol-induced keratinocyte damage	en_US
dc.type	Article	en_US