Abstract:
This study involves enzymatic extraction of fucoidan from Sargassum swartzii and further
purification via ion-exchange chromatography. The chemical and molecular characteristics of isolated
fucoidan is evaluated concerning its anti-inflammatory potential in RAW 264.7 macrophages under
LPS induced conditions. Structural properties of fucoidan were assessed via FTIR and NMR
spectroscopy. NO production stimulated by LPS was significantly declined by fucoidan. This was
witnessed to be achieved via fucoidan acting on mediators such as iNOS and COX-2 including
pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β), with dose dependent down-regulation. Further,
the effect is exhibited by the suppression of TLR mediated MyD88, IKK complex, ultimately hindering
NF-κB and MAPK activation, proposing its therapeutic applications in inflammation related disorders.
The research findings provide an insight in relation to the sustainable utilization of fucoidan from
marine brown algae S. swartzii as a potent anti-inflammatory agent in the nutritional, pharmaceutical,
and cosmeceutical sectors.
