Abstract:
Extensive polymorphism in the genes encoding for surface antigens of Plasmodium falciparum and Plasmodium vivax has been a serious impediment for malaria vaccine development. One such antigen is the merozoite surface protein-1 (MSP-1). The MSP-1 precursor after proteolytic cleavage generates a Cterminal fragment of 42 kDa (MSP-142), which subsequently produces 33 kDa (MSP-133) and 19 kDa (MSP-119) fragments. Since MSP-142 is currently being considered as a candidate for vaccine development against blood stage malaria it is important to catalogue the existing diversity in this antigeninnaturalP.vivaxinfections. Here weinvestigatedthelevelof geneticdiversityinthePvMSP-142 gene fragment in 95 single clone P. vivax infections in Sri Lanka. We observed that the PvMSP-119 fragment was highly conserved among these samples, whereas the PvMSP-133 fragment exhibited extensive diversity with 39 polymorphic amino acid positions (corresponding to 27 haplotypes, 19 of whichwereuniquetoSriLanka).Ofthese27PvMSP-142 haplotypes,24belongedtohypervariableregion (HVR) T1-T7 types, while 3 haplotypes were generated by interallelic recombination between T1/T3 (HVRT8-T9) and T2/T3 (HVRT10). In addition, we analysed 107 PvMSP-142 sequences (corresponding to 62 haplotypes, H28 to H89) deposited in the NCBI GenBank database from other regions of the world. Seventy-four of these correspond to 9 of the 10 HVR types (HVR-T7 was unique to Sri Lanka). Two novel HVR types, T11 and T12, with a double recombination between HVR-T1/T3 and HVRT6/T2, were derived from South America and Thailand, respectively. T cell epitope polymorphism arising due to nonsynonymous substitutions in PvMSP-133 may result in differential binding of the polymorphic peptides to class II MHC alleles, inducing different host immune responses. In conclusion, under low transmission and unstable malaria conditions prevalent in Sri Lanka, extensive allelic polymorphism was evident at PvMSP-133 due to recombination, mutation, and balancing selection. In contrast, PvMSP-119 is highly conserved, greatly enhancing its suitability as a malaria vaccine candidate.
