Purification of an acidic coniferin-hydrolysing β-glucosidase from developing xylem of Pinus banksiana

dc.contributor.author	Leinhos, Volker
dc.contributor.author	Udagama-Randeniya, Preethi V.
dc.contributor.author	Savidge, Rodney A.
dc.date.accessioned	2021-06-05T10:13:57Z
dc.date.available	2021-06-05T10:13:57Z
dc.date.issued	1994
dc.identifier.citation	38	en_US
dc.identifier.other	https://doi.org/10.1016/0031-9422(94)85053-4
dc.identifier.uri	http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5232
dc.description.abstract	Two cytosolic, acidic (pI 3.8) glycoproteins (Mr 110 000 and 90 000) from lignifying xylem of Pinus banksiana were electrophoretically isolated and confirmed by combined gas chromatography-mass spectrometry to be capable of hydrolysing E-coniferin to trans-coniferyl alcohol. These isoenzymes yielded identical N-terminal sequences having negligible homology with documented glucosidases, suggesting that they constitute a novel form of β-glucosidase.	en_US
dc.description.sponsorship	is work was supported by the Natural Sciences and Engineering Research Council of Canada and by Forestry Canada.	en_US
dc.language.iso	en	en_US
dc.publisher	Phytochemistry	en_US
dc.subject	Pinus banksiana	en_US
dc.subject	Gymnospermae	en_US
dc.subject	cambial growth	en_US
dc.subject	beta-glucosidase	en_US
dc.subject	lignin	en_US
dc.subject	secondary wall development	en_US
dc.subject	tracheary element differentiation	en_US
dc.subject	wood formation	en_US
dc.subject	xylogenesis	en_US
dc.title	Purification of an acidic coniferin-hydrolysing β-glucosidase from developing xylem of Pinus banksiana	en_US
dc.type	Article	en_US