Abstract:
Glycation of proteins results in the formation of Advanced Glycation End products
(AGEs) in the body and this is thought to be extremely important in the pathogenesis of
diabetes complications. Free radicals have been shown to participate in AGEs formation
and antioxidants/radical scavengers can inhibit this process. Rice bran (Rb) is a byproduct
of process of milling and reported to contain antioxidative compounds. Therefore, the
objective of the present study was to evaluate the effect of Rb on protein glycation
inhibitory activity (PGIA) and AGE cross linking breaking capacity (ACLBC).
Ethanolic extracts (70 %) of brans of 4 traditional rice (Oryza sativa) varieties (Masuran,
Dik wee, Goda Heeneti and Sudu Heeneti) were used in this study. Rb extracts of 1.56,
3.12, 6.25, 12.5, 25, 50 and 100 #g/ml were subjected to PGIA assay (n=6) according to
Matsuura et al., 2002 with some modifications. Rb extracts of 6.25, 12.5, 25, 50 and 100
#g/ml were used (n=6) to determine the ACLBC.
Rbs of all the selected traditional rice varieties showed very high PGIA and ACLBC in a
dose dependent manner. IC50 of protein glycation and ACLBC among different varieties
were statistically significant (P<0.05). Masuran had the highest PGIA (4.92 ± 0.24 #g/ml),
followed by Sudu Heeneti (5.79 ± 0.25 #g/ml), Dik wee (9.02 ± 0.23 #g/ml) and Goda
Heeneti (12.06 ± 0.36 #g/ml). Sudu Heeneti (44.25 ± 0.62 #g/ml) and Goda Heeneti
(46.07 ± 1.38 #g/ml) varieties had significantly high ACLBC. The EC50 values for
Masuran and Dik wee were 52.22 ± 1.11 #g/ml and 82.66 ± 3.34 #g/ml respectively.
It is concluded that, consumption of brans of selected Sri Lankan traditional rice may play
an important role in prevention of glycation-associated diseases. Further, this is the first
study to report the protein glycation inhibitory activity and AGE cross linking breaking
capacity in any varieties of rice worldwide
