Abstract:
The leaf, petiole, green stem, immature seed and anthers of Gossypium
hirsutum L. (cotton) were cultured on the basic Murashige and Skoog (1962)
medium (MS), supplemented with different types of growth regulators at
varying concentrations. They were cultured in 24 h light and dark conditions
separately. Callus formation was observed only on leaf and mature seed explants
cultured in light. The morphological and histological characters of the two
calluses varied. The calluses were transferred separately to basic MS liquid
medium supplemented with 2,4-Dichlorophenoxy acetic acid (2,4-D) and/or
Benzyl amino purine (BAP), to develop cell cultures. Cellular and quantitative
studies on the cell cultures enabled selection of the most suitable culture for
plant regeneration.
