Transmission Blocking Immunity in Plasmodium v/vax Malaria: Antibodies Raised against a Peptide Block Parasite Development in the Mosquito Vector

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dc.contributor.author Premawansa, Sunil
dc.date.accessioned 2012-04-04T11:34:54Z
dc.date.available 2012-04-04T11:34:54Z
dc.date.issued 1995
dc.identifier.citation J. Exp. Med Volume 181 January 1995 357-362 en_US
dc.identifier.uri http://archive.cmb.ac.lk:8080/xmlui/handle/70130/2303
dc.description.abstract One approach towards the development of a vaccine against malaria is to immunize against the parasite sexual stages that mediate transmission of the parasite from man to mosquito. Antibodies against these stages, ingested with the blood meal, inhibit the parasite development in the mosquito vector, constituting "transmission blocking immunity." Most epitopes involved in transmission-blocking immunity depend on the tertiary conformational structure of surface antigens. However, one of the transmission-blocking monodonal antibodies we have raised against Plasmodium vivax reacts with a linear epitope on both asexual stages and gametes. This monoclonal antibody (A12) is capable of totally blocking development of the parasite in the mosquito host when tested in membrane feeding assays with gametocytes from P. vivax-infected patients. Immune screening of a P. vivax Xgtll genomic expression library with A12 led to the isolation of a clone to which was mapped the six-amino acid epitope recognized by A12. Antisera raised in mice against a 12-mer synthetic peptide containing this epitope coupled to bovine serum albumin not only had high titers of antipeptide antibodies as measured by enzyme-linked immunosorbent assay, but in addition recognized the same 24- and 57-kD parasite components as A12 on Western blots and reacted with the parasite by immunofluorescence. When tested in membrane feeding assays, these antibodies have significant suppressive effects on parasite development in the mosquito. en_US
dc.language.iso en en_US
dc.title Transmission Blocking Immunity in Plasmodium v/vax Malaria: Antibodies Raised against a Peptide Block Parasite Development in the Mosquito Vector en_US
dc.type Journal full-text en_US


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