Abstract:
Morphogenesisof different explant types of 6-7 weeks old Solanum melongena L. variety " .
Padagoda was evaluated in response to different combinations of lAA and BAP in basal MS
medium. Leaves and stem pieces (hypocotyls) were found to be more responsive to hormone
combinationof 2 mg/L lAA with 1mg/LBAP containedMS mediumfor callusinduction.
Calli derivedfrom stem pieces regenerated shoots in the same regeneration medium while no
shootregenerationwas achieved calli derived from leaf explants cultured in all four types of
hormonecombinations,but root regenerationwas observed only calli derived from leaf explants
culturedonregenerationmedium containing 1mg/LBAP only.
Best shoot regeneration was obtained in media contained IAA in the presence of BAP with the
combination of 2 mg/L lAA with 1 mgIL BAP and 1 gmIL lAA with 1mg/L BAP. Direct shoot
regeneration was achieved in media containing high concentration of lAA where MS medium
consists of 1mgIL IAA only and MS medium consists of 2 mg/L lAA with 1 mg/L BAP.
An independent method of producing transgenic local eggplant (Solanum melongena L) variety
Padagoda was carried out by Agrobacterium mediated transformation.6- 7 weeks old leaf and
stem pieces were co-cultivated with Agrobacterium tumefaciens strain GV 1301 harboring the
binary vector pCAMBIA 1305.1 carrying a modified Bt gene construct cry 1C encoding a
lepidopteroninsect-specific Cry 1C toxin under the control of ST-LS1 promoter and NOS
terminator.This vector consists of reporter gene ~~glucuronidase(GUSPlus)and the marker gene
neomycin phosphotransferase (NPTII).Co-cultivated explants were introduced into callus
inductionmedium containing 2mg/1 lAA with 1 mg/L BAP and-250 mg/L cefotaxime. Callus
induced explants (stem pieces) were transferred into selection medium containing 2 mg/L lAA
with 1 mgIL BAP supplemented with 50 mgIL kanamycin. 33 kanamycin resistance explants
(stem pieces) out of 79 were obtained with 19 calli including 4 green calli.
