Abstract:
322 conjunctival and corneal swabs of both OPD and in ward patients with conjunctivitis
and corneal ulcers were processed. Also 31 samples of corneal button and 4 samples of
vitreous fluid from inward patients with corneal ulcers were procedded. All samples were
cultured on 3 agar media ( blood, chocolate, MacConkey) according to the standard
microbiological techniques. Identification was done by standered microbiological
techniques like gram stain, oxidase test, catalase test, etc. Confirmation of stero pneumoniae
was done by testing sensitivity to 5 microgram optochin disk and by the test of bile
solubility. Direct Gram stain of the swabs was done when it is possible and especially on the
request of the clinicians. All pneumococcal isolates were tested for MIC of penicillin and
were serogrouped.
