Abstract:
Many plant-based treatments are being recommended for the cancer patients by traditional medical practitioners
of Sri Lanka. Three such decoctions D1 (Terminalia bellerica, Terminalia chebula, Phyllanthus emblica and
detoxified Commiphora mukul), D2 (Terminalia bellerica, Terminalia chebula, Phyllanthus emblica, detoxified
Commiphora mukul, Smilax china and Nigella sativa) and D3 (Munronia Pumila, Azadirachta indica, Solanum
surattense, Solanum xanthocarpum, Rubia cordifolia, Picrorhiza kurroa, Trichosanthes cucumerina and
Pterocarpus santalinus) were selected to investigate their total polyphenol contents, antioxidant properties and
potential anticancer activities. The total phenolic contents of D1 and D2 were ~37 and 30% w/w gallic acid
equivalents, where as D3 contains a very low (6%) phenol content. Total free radical scavenging activity (DPPH
assay), reducing power and antilipid peroxidation activity (TBARS assay) of each decoction were investigated and
these values were compared with ascorbic acid and vitamin E. Decoction D1 and D2 showed higher antioxidant
activity and lower EC50 values than that of Decoction D3, which strongly associated with their total phenolic
content. The MTT assay and LDH assay were used to investigate antiproliferative and cytotoxic activities of these
decoctions against the human Rhabdomyosarcoma (RD) cells. The decoctions D1 and D2 showed strong inhibition of cell proliferation against RD cells, where as D3 did not show considerable activity. The chemo preventive and
therapeutic potential of the decoctions D1 and D2 can be explained to a certain extent by the results obtained
from this study.
