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http://archive.cmb.ac.lk:8080/xmlui/handle/70130/7410| Title: | Optimized PEI Mediated Reverse Transfection Methodology for Transient Transfection of HEK293 Cells |
| Authors: | Sirisena, K. Wijesinghe, K.J. |
| Keywords: | PEI Reverse Transfection HEK293 PEI: DNA Ratio |
| Issue Date: | 2024 |
| Publisher: | University of Colombo |
| Citation: | Sirisena, K., and Wijesinghe, K. J. (2024). Optimized PEI Mediated Reverse Transfection Methodology for Transient Transfection of HEK293 Cells. Proceedings: University of Colombo Annual Research Symposium 2024, p.284. |
| Abstract: | Non-viral vectors are increasingly used for gene delivery due to their safety and flexibility. Polyethyleneimine (PEI) is widely applied in transfection, but high PEI and DNA concentrations can induce cytotoxicity, necessitating careful optimization of the PEI ratio. ... |
| URI: | http://archive.cmb.ac.lk:8080/xmlui/handle/70130/7410 |
| Appears in Collections: | Department of Chemistry |
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