Please use this identifier to cite or link to this item: http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5558
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKariyawasam, K.K.G.D.U.L.-
dc.contributor.authorSelvapandiyan, A.-
dc.contributor.authorSiriwardana, H.V.Y.D.-
dc.contributor.authorDube, A.-
dc.contributor.authorKarunanayake, Panduka-
dc.contributor.authorSenanayake, S.A.S.C.-
dc.contributor.authorDey, R.-
dc.contributor.authorGannavaram, S.-
dc.contributor.authorNakhasi, H.L.-
dc.contributor.authorKarunaweera, N.D.-
dc.date.accessioned2021-07-29T07:56:02Z-
dc.date.available2021-07-29T07:56:02Z-
dc.date.issued2018-
dc.identifier.citation5. Kariyawasam, K. K. G. D. U. L., Selvapandiyan, A., Siriwardana, H. V. Y. D., Dube, A., Karunanayake, P., Senanayake, S. A. S. C., ... & Karunaweera, N. D. (2018). Dermotropic Leishmania donovani in Sri Lanka: visceralizing potential in clinical and preclinical studies. Parasitology, 145(4), 443-452.en_US
dc.identifier.urihttp://archive.cmb.ac.lk:8080/xmlui/handle/70130/5558-
dc.description.abstractThe visceralizing potential of apparently dermotropic Leishmania donovani in Sri Lanka (L. donovani-SL) was investigated through long-term follow-up of cutaneous leishmaniasis (CL) patients and in vivo and in vitro experimental infection models. CL patients (n = 250) treated effectively with intra-lesional antimony therapy were followed-up six monthly for 4 years. There was no clinical evidence of visceralization of infection (VL) during this period. Infection of BALB/c mice with L. donovani-SL (test) through intra-dermal route led to the development of cutaneous lesions at the site of inoculation with no signs of systemic dissemination, in contrast to the observations made in animals similarly infected with a visceralizing strain of L. donovani-1S (control). Cytokine (IL-10, IFN-γ) release patterns of splenocytes and lymph node cell cultures derived from mice primed with experimental infections (with either test or control parasites) revealed significantly high IFN-γ response associated with test mice with CL, while prominent IL-10 levels were observed in association with control mice with VL. Furthermore, diminished infection efficiency, intracellular growth and survival of L. donovani-SL parasites compared with L. donovani-1S were evident through in vitro macrophage infection experiments. These studies confirm, for the first time, the essential dermotropic nature of L. donovani-SL suggesting natural attenuation of virulence of local parasite strains.en_US
dc.language.isoenen_US
dc.publisherCambridge University Pressen_US
dc.subjectLeishmaniasisen_US
dc.subjectanimal modelsen_US
dc.subjectcytokinesen_US
dc.subjectpatient follow-upen_US
dc.subjectskin lesionsen_US
dc.subjectvirulenceen_US
dc.titleDermotropic Leishmania donovani in Sri Lanka: visceralizing potential in clinical and preclinical studiesen_US
dc.typeArticleen_US
Appears in Collections:Articles (local / International)

Files in This Item:
File Description SizeFormat 
6.pdf415.74 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.