Please use this identifier to cite or link to this item: http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5317
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dc.contributor.authorJayasinghe, J. D. H. E.
dc.contributor.authorElvitigala, D. A. S.
dc.contributor.authorWhang, I.
dc.contributor.authorNam, B. H.
dc.contributor.authorLee, J.
dc.date.accessioned2021-06-06T10:32:57Z
dc.date.available2021-06-06T10:32:57Z
dc.date.issued2015
dc.identifier.citationJayasinghe, J. D. H. E., Elvitigala, D. A. S., Whang, I., Nam, B. H., & Lee, J. (2015). Molecular characterization of two immunity-related acute-phase proteins: Haptoglobin and serum amyloid A from black rockfish (Sebastes schlegeli). Fish & shellfish immunology, 45(2), 680-688.en_US
dc.identifier.urihttp://archive.cmb.ac.lk:8080/xmlui/handle/70130/5317-
dc.description.abstractHaptoglobin (Hp) and serum amyloid A (SAA) are two vital proteins involved in inflammatory reactions and are classified as acute-phase proteins. They are released from hepatocytes under inflammatory conditions to protect healthy cells from being damaged by pathogens or from self-destructive mechanisms. In this study, a previously constructed black rockfish (Sebastes schlegeli) cDNA library was used to identify the full-length cDNA sequences of Hp and SAA homologs (RfHp and RfSAA, respectively) and characterize them at the molecular level. As expected, in silico analysis of these homologs showed the typical domain architectures of their known counterparts. Open reading frames of RfHp and RfSAA consisted of 942-bp and 313-bp DNA sequences, respectively. The derived polypeptide sequence of RfHp was composed of 313 amino acids (aa) with a predicted molecular weight of 34 kD, whereas RfSAA had a 121-amino acid sequence with a molecular weight of 13 kD. Phylogenetic analysis as well as pairwise sequence alignment results showed that RfHp was more closely related to Oreochromis mossambicus from an evolutionary perspective while RfSAA was closely related to the Epinephelus coioides ortholog. Although both genes were expressed ubiquitously in the tissues analyzed, they were particularly expressed in liver tissue, suggesting their origin in hepatocytes. Quantitative real-time PCR analysis indicated that both RfHp and RfSAA were significantly up-regulated by both bacterial and viral stimulation in liver tissue, affirming their putative importance in the acute phase of first-line host immune defenses
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectHaptoglobinen_US
dc.subjectSerum amyloid Aen_US
dc.subjectBlack rockfishen_US
dc.subjectImmune challengeen_US
dc.subjectTranscriptional responseen_US
dc.titleMolecular characterization of two immunity - related acute- phase proteins : Haptoglobin and serum amyloid A from black rockfish (Sebastes schlegeli)en_US
dc.typeArticleen_US
Appears in Collections:Department of Basic Sciences & Social Sciences

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