Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorElvitigala, D. A. S.
dc.contributor.authorWhang, I.
dc.contributor.authorJung, H. B.
dc.contributor.authorLim, B. S.
dc.contributor.authorNam, B. H.
dc.contributor.authorLee, J.
dc.identifier.citationElvitigala, D. A. S., Whang, I., Jung, H. B., Lim, B. S., Nam, B. H., & Lee, J. (2015). Molecular delineation of a caspase 10 homolog from black rockfish (Sebastes schlegelii) and its transcriptional regulation in response to pathogenic stress. Gene, 570(2), 288-294.en_US
dc.description.abstractCaspase 10 is an initiator caspase in death cascades of death receptor mediated apoptotic signaling. We identified and molecularly characterized a novel homolog of caspase 10 from black rockfish (Sebastes schlegelii) and designated as RfCasp10. The complete coding region of RfCasp10 was found to consist of 1659 bps, encoding a 553 amino acid protein with a predicted molecular mass of 61.7 kDa. The characteristic caspase family domain architecture, including death effecter domains (DEDs), was clearly identified in RfCasp10. Moreover, the RfCasp10 gene was found to contain 13 exons. Our pairwise sequence alignment confirmed the prominent sequence similarity of RfCasp10 with its fish homologs, and phylogenetic reconstruction affirmed its homology and substantial evolutionary relationship with known caspases 10 similitudes, in particular with those of teleosts. As detected by qPCR, RfCasp10 was markedly expressed in blood tissues under physiological conditions, whereas its expression was found to be upregulated under pathogenic stress, elicited by Streptococcus iniae and polyinosinic:polycytidylic acid in blood, liver, and spleen tissues. Collectively, our study suggests the plausible elicitation of RfCasp10 mediated apoptosis in immune relevant tissues of black rockfish as a host immune response to a bacterial or viral infection
dc.subjectCaspase 10en_US
dc.subjectBlack rockfishen_US
dc.subjectGenomic geneen_US
dc.subjectIn silico analysisen_US
dc.subjectTranscriptional analysisen_US
dc.titleMolecular delineation of a caspase 1 homolog from black rockfish (sebastes schlegelii) and its transcriptional regulation in response to pathogenic stressen_US
Appears in Collections:Department of Basic Sciences & Social Sciences

Files in This Item:
File Description SizeFormat 
15-11.pdf2.29 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.