Please use this identifier to cite or link to this item: http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5310
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dc.contributor.authorPriyathilaka, T.T.
dc.contributor.authorKim, Y
dc.contributor.authorUdayantha, H. M. V
dc.contributor.authorLee, S.
dc.contributor.authorHerath, H. M. L. P. B.
dc.contributor.authorLakmal, H. C
dc.contributor.authorElvitigala, D.A.S.
dc.contributor.authorUmasuthan, N.
dc.contributor.authorGodahewa, G.I.
dc.contributor.authorKang, S.I.
dc.contributor.authorJeong, H.B
dc.contributor.authorKim, S,K.
dc.contributor.authorKim, D.J
dc.date.accessioned2021-06-06T09:26:12Z
dc.date.available2021-06-06T09:26:12Z
dc.date.issued2016
dc.identifier.citationPriyathilaka, T. T., Kim, Y., Udayantha, H. M. V., Lee, S., Herath, H. M. L. P. B., Lakmal, H. C., ... & Lim, B. S. (2016). Identification and molecular characterization of peroxiredoxin 6 from Japanese eel (Anguilla japonica) revealing its potent antioxidant properties and putative immune relevancy. Fish & shellfish immunology, 51, 291-302.en_US
dc.identifier.urihttp://archive.cmb.ac.lk:8080/xmlui/handle/70130/5310-
dc.description.abstractPeroxiredoxins (Prdx) are thiol specific antioxidant enzymes that play a pivotal role in cellular oxidative stress by reducing toxic peroxide compounds into nontoxic products. In this study, we identified and characterized a peroxiredoxin 6 counterpart from Japanese eel (Anguilla japonica) (AjPrdx6) at molecular, transcriptional and protein level. The identified full-length coding sequence of AjPrdx6 (669 bp) coded for a polypeptide of 223 aa residues (24.9 kDa). Deduced protein of AjPrdx6 showed analogy to characteristic structural features of 1-cysteine peroxiredoxin sub-family. According to the topology of the generated phylogenetic reconstruction AjPrdx6 showed closest evolutionary relationship with Salmo salar. As detected by Quantitative real time PCR (qPCR), AjPrdx6 mRNA was constitutively expressed in all the tissues examined. Upon the immune challenges with Edwardsiella tarda, lipopolysaccharides and polyinosinic:polycytidylic acid, expression of AjPrdx6 mRNA transcripts were significantly induced. The general functional properties of Prdx6 were confirmed using purified recombinant AjPrdx6 protein by deciphering its potent protective effects on cultured vero cells (kidney epithelial cell from an African green monkey) against H2O2-induced oxidative stress and protection against oxidative DNA damage elicited by mixed function oxidative (MFO) system. Altogether, our findings suggest that AjPrdx6 is a potent antioxidant protein in Japanese eels and its putative immune relevancy in pathogen stress mounted by live-bacteria or pathogen associated molecular patterns (PAMPs)
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectPeroxiredoxin 6en_US
dc.subjectAnguilla japonicaen_US
dc.subjectAntioxidanten_US
dc.subjectOxidative stressen_US
dc.titleIdentification and molecular characterization of peroxiredoxin 6 from japanese eel (Anguilla japonica) revealing its potent antioxidant properties and putative immune relevancyen_US
dc.typeArticleen_US
Appears in Collections:Department of Basic Sciences & Social Sciences

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