Please use this identifier to cite or link to this item: http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5307
Title: Identification and molecular profiling of DC - SIGN - like from big belly seahorse (Hippocampus abdominalis) inferring its potential relevancy in host immunity
Authors: Jo, E.
Elvitigala, D. A. S
Wan, Q.
Oh, M.
Oh, C.
Lee, J.
Keywords: Big belly seahorse
DC-SIGN
Immune stimulation
Transcription analysis
Bacterial agglutination activity
Issue Date: 2017
Publisher: Elsevier
Citation: Jo, E., Elvitigala, D. A. S., Wan, Q., Oh, C., & Lee, J. (2017). Identification and molecular profiling of DC-SIGN-like from big belly seahorse (Hippocampus abdominalis) inferring its potential relevancy in host immunity. Developmental & Comparative Immunology, 77, 270-279.
Abstract: Dendritic-cell-specific ICAM-3-grabbing non-integrin (DC-SIGN) is a C-type lectin that functions as a pattern recognition receptor by recognizing pathogen-associated molecular patterns (PAMPs). It is also involved in various events of the dendritic cell (DC) life cycle, such as DC migration, antigen capture and presentation, and T cell priming. In this study, a DC-SIGN-like gene from the big belly seahorse Hippocampus abdominalis (designated as ShDCS-like) was identified and molecularly characterized. The putative, complete ORF was found to be 1368 bp in length, encoding a protein of 462 amino acids with a molecular mass of 52.6 kDa and a theoretical isoelectric point of 8.26. The deduced amino acid sequence contains a single carbohydrate recognition domain (CRD), in which six conserved cysteine residues and two Ca2þ-binding site motifs (QPN, WND) were identified. Based on pairwise sequence analysis, ShDCSlike exhibits the highest amino acid identity (94.6%) and similarity (97.4%) with DC-SIGN-like counterpart from tiger tail seahorse Hippocampus comes. Quantitative real-time PCR revealed that ShDCS-like mRNA is transcribed universally in all tissues examined, but with abundance in kidney and gill tissues. The basal mRNA expression of ShDCS-like was modulated in blood cell, kidney, gill and liver tissues in response to the stimulation of healthy fish with lipopolysaccharides (LPS), Edwardsiella tarda, or Streptococcus iniae. Moreover, recombinant ShDCS-like-CRD domain exhibited detectable agglutination activity against different bacteria. Collectively, these results suggest that ShDCS-like may potentially involve in immune function in big belly seahorses.
URI: http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5307
Appears in Collections:Department of Basic Sciences & Social Sciences

Files in This Item:
File Description SizeFormat 
17-1.pdf3.3 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.