Please use this identifier to cite or link to this item: http://archive.cmb.ac.lk:8080/xmlui/handle/70130/5267
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dc.contributor.authorAbeysinghe, Saman-
dc.contributor.authorAbeysinghe, Pushpa Damayanthi-
dc.contributor.authorKanatiwela-de Silva, Chamini-
dc.contributor.authorUdagama, Preethi-
dc.contributor.authorWarawichanee, Kanjana-
dc.contributor.authorAljafar, Naofel-
dc.contributor.authorKawicha, Praphat-
dc.contributor.authorDickinson, Matt-
dc.date.accessioned2021-06-05T10:29:16Z-
dc.date.available2021-06-05T10:29:16Z-
dc.date.issued2016-
dc.identifier.citation16en_US
dc.identifier.otherhttps://doi.org/10.1094/PDIS-02-16-0244-RE-
dc.identifier.urihttp://archive.cmb.ac.lk:8080/xmlui/handle/70130/5267-
dc.description.abstractPhytoplasmas that infect gramineous plants, including Napier grass stunt, sugarcane whiteleaf, sugarcane grassy shoot, and Bermuda grass whiteleaf, have been classified into two closely related groups, 16SrXI and 16SrXIV, based on the 16S ribosomal RNA (rRNA) gene. Subsequently, phytoplasmas associated with coconut and Areca palm in southern India and Sri Lanka have been added into the 16SrXI group. However, the 16S rRNA gene gives relatively poor resolution between these phytoplasmas. In this study, a new set of universal phytoplasma primers that amplify approximately 1 kb of the leucyl transfer RNA synthetase (leuS) gene have been validated on a broad range of phytoplasma taxonomic groups. These have been used along with partial sequences of the secA gene to clarify the taxonomic classification of 16SrXI and 16SrXIV phytoplasmas. Based on this data, the sugarcane whiteleaf and grassy shoot phytoplasmas appear to be the same phytoplasma. The Napier grass stunt phytoplasma forms a distinct group from the Bermuda grass whiteleaf and sugarcane phytoplasmas, suggesting that Napier grass stunt should be in its own ‘Candidatus Phytoplasma sp.’. The phytoplasmas associated with coconut and arecanut in southern India and Sri Lanka, which are in the same 16SrXI group, appear in different groups based on secA analysis.en_US
dc.description.sponsorshipCommonwealth Scholarshipen_US
dc.language.isoenen_US
dc.publisherAPS publicationen_US
dc.subject6SrXI and 16SrXIV Phytoplasmasen_US
dc.subjectGramineous Plantsen_US
dc.subjectMultilocus Sequence Typingen_US
dc.titleFree Access icon Refinement of the Taxonomic Structure of 16SrXI and 16SrXIV Phytoplasmas of Gramineous Plants using Multilocus Sequence Typingen_US
dc.typeArticleen_US
Appears in Collections:Department of Zoology

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