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Title: Anti-Inflammatory Effects of Sulfated Polysaccharide from Sargassum swartzii in Macrophages via Blocking TLR/NF-Kb Signal Transduction
Authors: Jayawardena, Thilina U.
Sanjeewa, K. K. Asanka
Nagahawatta, D. P.
Lee, Hyo-Geun
Lu, Yu-An
Vaas, A. P. J. P.
Abeytunga, D. T. U.
Nanayakkara, C.M.
Lee, Dae-Sung
Jeon, You-Jin
Keywords: Sargassum swartzii; TLR/MyD88; NF-κB; MAPK; fucoidan
Issue Date: 2020
Publisher: Marine Drugs
Citation: Thilina U. Jayawardena, K. K. Asanka Sanjeewa , D. P. Nagahawatta, Hyo-Geun Lee, Yu-An Lu, A. P. J. P. Vaas, D. T. U. Abeytunga, C. M. Nanayakkara, Dae-Sung Lee, and You-Jin Jeon (2020). Anti-Inflammatory Effects of Sulfated Polysaccharide from Sargassum swartziiin Macrophages via Blocking TLR/NF-Kb Signal Transduction, Marine Drugs 2020 18, 601, 1-20
Abstract: This study involves enzymatic extraction of fucoidan from Sargassum swartzii and further purification via ion-exchange chromatography. The chemical and molecular characteristics of isolated fucoidan is evaluated concerning its anti-inflammatory potential in RAW 264.7 macrophages under LPS induced conditions. Structural properties of fucoidan were assessed via FTIR and NMR spectroscopy. NO production stimulated by LPS was significantly declined by fucoidan. This was witnessed to be achieved via fucoidan acting on mediators such as iNOS and COX-2 including pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β), with dose dependent down-regulation. Further, the effect is exhibited by the suppression of TLR mediated MyD88, IKK complex, ultimately hindering NF-κB and MAPK activation, proposing its therapeutic applications in inflammation related disorders. The research findings provide an insight in relation to the sustainable utilization of fucoidan from marine brown algae S. swartzii as a potent anti-inflammatory agent in the nutritional, pharmaceutical, and cosmeceutical sectors.
Appears in Collections:Department of Plant Sciences

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