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|Title:||Purification of an acidic coniferin-hydrolysing β-glucosidase from developing xylem of Pinus banksiana|
Udagama-Randeniya, Preethi V.
Savidge, Rodney A.
secondary wall development
tracheary element differentiation
|Abstract:||Two cytosolic, acidic (pI 3.8) glycoproteins (Mr 110 000 and 90 000) from lignifying xylem of Pinus banksiana were electrophoretically isolated and confirmed by combined gas chromatography-mass spectrometry to be capable of hydrolysing E-coniferin to trans-coniferyl alcohol. These isoenzymes yielded identical N-terminal sequences having negligible homology with documented glucosidases, suggesting that they constitute a novel form of β-glucosidase.|
|Appears in Collections:||Department of Zoology|
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