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|Title:||Plasmadium vivax: Cloning and Expression of a Major Blood-Stage . Surface Antigen|
|Authors:||Del Portillo, Hernando A.|
Mattie, Denise M.
Udagama, Preethi V.
Mendis, Kamini N.
David, Peter H
|Keywords:||Blood-stage surface antigen|
|Abstract:||Plasmodium vivax is a highly prevalent malaria pathogen of man; the following report is the first to describe the cloning and expression of a major asexual erythrocytic stage antigen of this species. The screening of a genomic DNA expression lilrary with a monoclonal antibody directed against a 200-kDa surface component (Pv200) of the more mature schizonts of P. vivax led to the selection of a recombinant bacterial clone which produced a fusion protein. Mouse and rabbit immune sera raised against the purified fusion protein recognized the 200-kDa parasite antigen on Western blots and reacted with the surface of segmenters by immunofluorescence. Sequencing of the 1.9-kb P. vivax DNA insert coding for this fusion protein revealed a 4547Vo homology at the nucleotide level with the P . falciparum gene of a parasite surface antigen,Pflgs, which has been shown to be a promising candidate for a malaria vaccine in primates and in man|
|Description:||Plasmodium vivax,' Blood-stage surface antigen; DNA sequence; Deoxyribonucleic acid (DNA); Indirect immunofluorescence antibody test (IFAT); Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE); Kilobase (kb); Kilo daltons (kDa); Molecular weight range (M); Base pair (bp).|
|Appears in Collections:||Department of Zoology|
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