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|FTIR characterization and antioxidant activity of water soluble crude polysaccharides of Sri Lankan marine algae
Fernando, I. P. Shanura
Sanjeewa, K. K. Asanka
Samarakoon, Kalpa W.
Lee, Won Woo
Gunasekara, U. K. D. S. S.
de Silva, E. D.
|Chnoospora minima; electron spin resonance; FTIR analysis; polysaccharides; Sri Lankan
|Algae 2017, 32(1): 75-86
|Polysaccharides of marine algae exhibit different structural characteristics and interesting biological functions. In this study, crude polysaccharides (CP) of eleven Sri Lankan marine algae obtained through hot water extraction and ethanol precipitation were investigated for DPPH, alkyl, and hydroxyl radical scavenging activities using electron spin resonance spectrometry and for intracellular reactive oxygen species scavenging activity in the Chang liver cell line. Characterization of CPs was done by Fourier transform infrared (FTIR) spectroscopy and by analysis of the monosaccharide composition. Time-dependent density functional theory quantum-chemical calculations at the RB3LYP/6-31G(d,p) level for constructed dimeric units of the corresponding polysaccharides were used to resolve the FTIR spectra. CPs from Chnoospora minima showed the highest DPPH and alkyl radical scavenging activities and higher intracellular reactive oxygen species scavenging effects for both AAPH and H2O2 induced ROS production in “Chang” cells. The major polysaccharide constituent in C. minima CP was identified as fucoidan and it displayed a higher sulfate content. The degree of sulfation of these polysaccharides suggests a positive correlation with the observed antioxidant properties.
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|Department of Chemistry
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