Please use this identifier to cite or link to this item: http://archive.cmb.ac.lk:8080/xmlui/handle/70130/377
Full metadata record
DC FieldValueLanguage
dc.contributor.authorPremaratne, P. H.
dc.contributor.authorAravinda, B. R.
dc.contributor.authorEscalante, A. A.
dc.contributor.authorUdagama-Randeniya, P. V.
dc.date.accessioned2011-11-15T10:27:14Z
dc.date.available2011-11-15T10:27:14Z
dc.date.issued2010
dc.identifier.citationAnnual Research Proceedings, University of Colombo held on 12th and 13th May 2010en_US
dc.identifier.urihttp://archive.cmb.ac.lk:8080/xmlui/handle/70130/377
dc.description.abstractPhylogenetic relationship of the region II of the P. vivax Duffy Binding Protein gene (PvdbpII), a prime vaccine candidate against blood stage of vivax malaria, was analysed between parasite isolates from Sri Lanka and from different geographical areas, worldwide. A gene tree was derived from the aligned nucleotide sequences of a 672 bp region, using the Neighbour-Joining method with 1000 bootstrap replicates, the Tamura's three-parameter distance model as implemented in the MEGA version 4.0 programme. One hundred and seventy five PvdbpII sequences found in the GenBank were compiled and compared to 100 Sri Lankan (SL) sequences. The worldwide sequences included those from the reference Salvador-I strain, single sequences each from Vietnam (VIAT), Indonesia (INDO) and India (INDI) and 13, 17, 30 and 111 sequences from South Korea (SK), Colombia (COL), Thailand (Thai) and Papua New Guinea (PNG), respectively. Twenty three different groups in all were evident in the gene tree. SL isolates were grouped in 6 different clades (A-G). A majority of these concentrated amongst 3 different clades (A, B and C), with no clustering detected according to the sample collection sites within the country. A single clade (D), exclusively contained SL isolates, proving evidence for geographical isolation. Two other clades (A & F) contained isolates from SL in combination with THAI and SK, respectively. The close phylogeny of the SL sequences with world wide ones were more clearly evident in the gene tree by SL isolates grouping with those from PNG, THAI, COL, INDO and VIAT in clade B, and with those from PNG, THAI, COL and from INDI with the reference strain Sal-1 in clade E. Interestingly, both these groups lacked SK isolates. The Sri Lankan PvdbpII sequences thus appeared to represent a sample from the PvdbpII worldwide genetic diversity, rather than from any particular lineage
dc.language.isoenen_US
dc.publisherUniversity of Colomboen_US
dc.titlePhylogenetic relationship of Plasmodium vivax duffy binding protein gene between Sri Lankan and world wide isolatesen_US
dc.typeResearch abstracten_US
Appears in Collections:Science

Files in This Item:
File Description SizeFormat 
Abstracts (dragged) 9.pdf93.52 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.