Please use this identifier to cite or link to this item: http://hdl.handle.net/70130/2476
Title: IN VITRO CALLUS AND C E L L CULTURES OF GOSSYPIUM HIRSUTUM L. (COTTON)
Authors: Hirimburegama, Kshanika
Gamage, N.
Issue Date: 1994
Citation: ./. Natn. Sri. Coun. Sri Lanka 1994 22(4): 305
Abstract: The leaf, petiole, green stem, immature seed and anthers of Gossypium hirsutum L. (cotton) were cultured on the basic Murashige and Skoog (1962) medium (MS), supplemented with different types of growth regulators at varying concentrations. They were cultured in 24 h light and dark conditions separately. Callus formation was observed only on leaf and mature seed explants cultured in light. The morphological and histological characters of the two calluses varied. The calluses were transferred separately to basic MS liquid medium supplemented with 2,4-Dichlorophenoxy acetic acid (2,4-D) and/or Benzyl amino purine (BAP), to develop cell cultures. Cellular and quantitative studies on the cell cultures enabled selection of the most suitable culture for plant regeneration.
URI: http://archive.cmb.ac.lk:8080/research/handle/70130/2476
Appears in Collections:Department of Plant Sciences

Files in This Item:
File Description SizeFormat 
2-1-1-34.pdf52.39 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.